Effects of di-isononyl phthalate (DINP) on peroxisomal markers in the marmoset - DINP is not a peroxisome proliferator
Hall, M; Matthews, A; Webley, L; Harling, R
HERO ID
679627
Reference Type
Journal Article
Year
1999
Language
English
PMID
| HERO ID | 679627 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Effects of di-isononyl phthalate (DINP) on peroxisomal markers in the marmoset - DINP is not a peroxisome proliferator |
| Authors | Hall, M; Matthews, A; Webley, L; Harling, R |
| Journal | Journal of Toxicological Sciences |
| Volume | 24 |
| Issue | 3 |
| Page Numbers | 237-244 |
| Abstract | In the present study the systemic toxic potential of di-isononyl phthalate (DINP) was assessed in a 13-week study in marmosets. Particular attention was given to its potential for hepatic peroxisome proliferation. Three groups of four male and four female marmosets received DINP, by oral gavage administration, at dosages of 100, 500 or 2500 mg/kg/day for 13 weeks. A fourth group served as a concurrent Control group and received the vehicle (1% methylcellulose and 0.5% Tween) only. A fifth group received clofibrate at a dosage of 500 mg/kg/day to provide a positive Control for liver peroxisome activity. At the end of the treatment period, the animals were killed and their livers were removed. 3000 x g supernatant and microsomal subcellular fractions were prepared from homogenised liver by differential centrifugation. The peroxisomal marker enzyme activity, cyanide-insensitive palmitoyl CoA oxidase, was assayed in the former, while cytochrome P450 concentration and lauric acid 11- and 12-hydroxylase activities (selective for CYP2E1 and 4A, respectively) were assayed in the microsomes. No statistically significant changes were seen in any of these parameters measured following DINP treatment, compared with the Control. Clofibrate treatment resulted in an approximately 100% increase (p < 0.01) in both male and female marmoset cyanide-insensitive palmitoyl CoA oxidase activity and a similar increase (p < 0.05) in male (only) lauric acid 11-hydroxylase activity. No other changes were statistically significant at the 5% level. These data provide no evidence that DINP was acting as a peroxisome proliferator when administered to marmosets under the conditions of the study |
| Doi | 10.2131/jts.24.3_237 |
| Pmid | 10478338 |
| Url | https://search.proquest.com/docview/1468879541?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Alkane 1-Monooxygenase; Animals; Biological Markers; Callithrix; Cell Fractionation; Centrifugation; chemistry; Clofibrate; Cytochrome P-450 CYP2E1; Cytochrome P-450 Enzyme System; Diethylhexyl Phthalate; drug effects; enzymology; Female; Kidney; Liver; Male; Membrane Proteins; metabolism; Microbodies; Microsomes; Microsomes,Liver; Mixed Function Oxygenases; Organ Size; Oxidoreductases; Oxygenases; pathology; Peroxisome Proliferators; Phthalic Acids; Proteins; Salivary Glands; Subcellular Fractions; toxicity; Toxicity Tests; Weight Gain |
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