Effect of phthalate esters and alkylphenols on steroidogenesis in human adrenocortical H295R cells
Nakajin, S; Shinoda, S; Ohno, S; Nakazawa, H; Makino, T
HERO ID
679781
Reference Type
Journal Article
Year
2001
Language
English
PMID
| HERO ID | 679781 |
|---|---|
| In Press | No |
| Year | 2001 |
| Title | Effect of phthalate esters and alkylphenols on steroidogenesis in human adrenocortical H295R cells |
| Authors | Nakajin, S; Shinoda, S; Ohno, S; Nakazawa, H; Makino, T |
| Journal | Environmental Toxicology and Pharmacology |
| Volume | 10 |
| Issue | 3 |
| Page Numbers | 103-110 |
| Abstract | The authors investigated the inhibitory effects of phthalate esters and alkylphenols on steroidogenesis by human adrenocortical H295R cells, a model of human steroidogenic cells. Dicyclohexyl phthalate (DCHP) at a concn. of 30 ?M produced a significant decrease in the dibutyryl cAMP-stimulated cortisol secretion (76% redn.). 4-t-Pentylphenol (4-t-PP), 4-t-octylphenol (4-t-OP) and 4-nonylphenol (4-NP) also produced significant decreases in the dibutyryl cAMP-stimulated cortisol secretion by 58, 34 and 40%, resp. at 50 ?M. Redns. in cortisol secretion by these chems. were dose-dependent. To elucidate the inhibitory effects of DCHP, 4-t-PP, 4-t-OP and 4-NP on cortisol secretion from H295R cells, the effects of these chems. on various steroidogenic enzymes, such as C20,22-lyase (CYP11A), 3?-hydroxysteroid dehydrogenase type II (3?-HSDII), 17?-hydroxylase/C17,20-lyase (CYP17), 21-hydroxylase (CYP21B) and 11?-hydroxylase (CYP11B1), were investigated. DCHP significantly inhibited CYP21B activity at 25 ?M. 4-t-OP strongly inhibited CYP11A activity at 12.5 and 25 ?M, and inhibited CYP17 and CYP21B at 25 ?M. Similarly, 4-NP inhibited CYP11A at 25 ?M and strongly inhibited CYP17 and CYP21B at 12.5 and 25 ?M. Neither DCHP nor any of the alkylphenols tested altered 3?-HSDII activity. |
| Doi | 10.1016/S1382-6689(01)00089-8 |
| Pmid | 21782564 |
| Wosid | WOS:000170659600005 |
| Url | https://search.proquest.com/docview/14580951?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000170659600005 |
| Is Public | Yes |
| Language Text | English |
| Keyword | adrenocortical H259R cells; steroidogenesis; phthalate esters; alkylphenols; enzyme inhibition |
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