Developmental toxic effects of di-iso-butyl phthalate administered by gavage to rats
Saillenfait, AM; Sabate, JP; Gallissot, F
HERO ID
680388
Reference Type
Journal Article
Subtype
Abstract
Year
2005
Language
English
| HERO ID | 680388 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 2005 |
| Title | Developmental toxic effects of di-iso-butyl phthalate administered by gavage to rats |
| Authors | Saillenfait, AM; Sabate, JP; Gallissot, F |
| Journal | Toxicology |
| Volume | 213 |
| Issue | 3 |
| Page Numbers | 231-232 |
| Abstract | Despite its wide use in the manufacture of plastics, there is no published information on the developmental toxic potential of di-iso-butyl phthalate (DIBP). Its isomer, di-n-butyl phthalate, have been associated with developmental toxic effects in rodents, especially on the male reproductive system. This study was conducted to assess the effects of orally administered DIBP on the embryonic and fetal development in rats. Groups of 10–14 pregnant Sprague–Dawley rats were given DIBP by gavage (5 ml/kg), on Gestation Day (GD) 6 to 20, at doses of 0 (vehicle: olive oil), 250, 500, 750, or 1000 mg/kg/day. Maternal body weights and clinical signs were recorded. Dams were euthanized on GD 21, and the uterine contents were evaluated for the number of implantation sites, resorptions, fetal deaths, and live fetuses. All live fetuses were weighed and submitted to external examination. They were fixed in 10% neutral-buffered formalin, and internal gross examination of the reproductive tract was performed under a dissecting microscope. Sex was determined by examination of gonads. Maternal body weight gain was significantly depressed at 750 and 1000 mg/kg during the last days of treatment (GD 15–18, and GD 18–21). However, the weight gains during GD 6–21 corrected for uterine weight were comparable across groups (Table 1: see text). A significant increase in the mean percentage of resorptions per litter was observed in a doserelated manner at 500, 750 and 1000 mg/kg. The fetal body weight was significantly decreased at 750 and 1000 mg/kg. External malformations were observed in two fetuses from two different litters at 750 mg/kg: one fetus showed general oedema, and another exhibited multiple malformations including anal atresia, small genital tubercle, ectrodactyly, shortened hindlimbs. Because of their single occurrence and in the absence of a dose–response pattern, they could not be conclusively attributed to DIBP. Undescended testes were apparent in 56 and 70% of the male fetuses at 750 and 1000 mg/kg, respectively. There was no significant difference in the fetal sex ratio between the vehicle controls and the DIBP groups. This study demonstrates the developmental toxicity of DIBP administered to rats, by gavage, throughout the embryonic and fetal period. Further experiments are needed to characterize the full scale of DIBP developmental toxicity. |
| Doi | 10.1016/j.tox.2005.05.016 |
| Wosid | WOS:000232573800050 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Warwick, UK |
| Conference Name | BTS Annual Congress |
| Conference Date | March 21-23,2005 |
| Comments | Source: Web of Science 000232573800050 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Pregnancy; Rats; Animals; Female; Rats, Sprague-Dawley; Gestational Age; Dibutyl Phthalate/*analogs & derivatives/*TOXICITY; Administration, Oral; Embryo Loss/*CHEMICALLY INDUCED; Fetal Development/*DRUG EFFECTS; Abnormalities/*ETIOLOGY; 84-74-2; 84-69-5 |
| Is Qa | No |