UV-FIA: UV-induced fluoro-immunochemical assay for ultra-trace detection of PETN, RDX, and TNT
Chaudhary, S; Sonkusre, P; Chopra, A; Bhasin, KK; Suri, CR; ,
| HERO ID | 6889772 |
|---|---|
| In Press | No |
| Year | 2019 |
| Title | UV-FIA: UV-induced fluoro-immunochemical assay for ultra-trace detection of PETN, RDX, and TNT |
| Authors | Chaudhary, S; Sonkusre, P; Chopra, A; Bhasin, KK; Suri, CR; , |
| Journal | Analytica Chimica Acta |
| Volume | 1077 |
| Page Numbers | 266-272 |
| Abstract | Fluorescence quenching based immunoassay format for the detection of a trace amount of some nitro-explosives with a high degree of selectivity is reported in this study. The immunoassay comprises anti-explosive antibodies functionalized microtitre strips specific to the targeted explosives, pentaerythritol tetranitrate (PETN), 1,3,5-trinitroperhydro-1,3,5-triazine (RDX), and 2,4,6-trinitrotoluene (TNT). UV induced photolysis of nitro-explosive bound to targeted antibodies generates primarily nitrite ions which after the quick reaction with the detector molecule, 2,3-diaminonaphthalene (DAN), a fluorophore, quenches its fluorescence intensity, however, proportionately undergo cyclization to produce a highly fluorescent product, 2,3-naphthotriazole (NAT). The synthesized product, NAT, was verified using various chromatographic and spectrophotometric techniques. This newly developed antibody-based detection method, utilizing DAN dye, demonstrated a high selectivity towards PETN, RDX, and TNT. This method can be used as an economical testing kit for direct quantification of explosives, implying the great potential for quick, low-cost trace detection of explosives. |
| Doi | 10.1016/j.aca.2019.05.048 |
| Pmid | 31307718 |
| Wosid | WOS:000475299800025 |
| Url | https://linkinghub.elsevier.com/retrieve/pii/S0003267019306452 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | 2,3-Diaminonaphthalene (DAN); 2,3-Naphthotriazole (NAT); Charge-transfer fluorophore; Fluorescence quenching; Immunoassay; Nitro-explosives |