Vanadium redox cycling, lipid peroxidation and co-oxygenation of benzo(a)pyrene-7,8-dihydrodiol
Byczkowski, JZ; Kulkarni, AP
HERO ID
758664
Reference Type
Journal Article
Year
1992
Language
English
PMID
| HERO ID | 758664 |
|---|---|
| In Press | No |
| Year | 1992 |
| Title | Vanadium redox cycling, lipid peroxidation and co-oxygenation of benzo(a)pyrene-7,8-dihydrodiol |
| Authors | Byczkowski, JZ; Kulkarni, AP |
| Journal | Biochimica et Biophysica Acta |
| Volume | 1125 |
| Issue | 2 |
| Page Numbers | 134-141 |
| Abstract | Mechanism of lipid peroxidation triggered by vanadium in human term placental microsomes was reinvestigated in vitro. Production of lipid peroxyl radicals was estimated from co-oxygenation of benzo(a)pyrene and benzo(a)pyrene-7,8-dihydrodiol. Vanadyl(IV), but not vanadate(V) caused a dose-dependent co-oxygenation. Vanadate(V) required the presence of reduced nicotinamide adenine dinucleotide phosphate to trigger co-oxygenation of benzo(a)pyrene-7,8-dihydrodiol. To determine the role of pre-formed lipid hydroperoxides, the results obtained with partially peroxidized linoleic acid were compared with those of fresh linoleate. Superoxide dismutase inhibited the co-oxygenation of reaction when fresh linoleic acid was used. To further characterize the role of superoxide anion-radical in the vanadium redox cycling, the increase of optical density of vanadate(V) dissolved in Tris buffer was measured at 328 nm during the addition of KO2. The rate of this reaction producing peroxy-vanadyl complex was decreased by superoxide dismutase, especially, in the presence of catalase. It is suggested that vanadium catalyzes two separate processes, both leading to enhanced lipid peroxidation: (i) initiation, dependent on superoxide and triggered by peroxy-vanadyl; (ii) propagation, dependent on pre-formed lipid hydroperoxide not sensitive to superoxide dismutase. It is postulated that the vanadium-triggered initiation of lipid peroxidation may be crucial for toxicity in organs with limited endogenous lipid peroxidation. |
| Doi | 10.1016/0005-2760(92)90037-V |
| Pmid | 1315157 |
| Wosid | WOS:A1992HT30800002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026528023&doi=10.1016%2f0005-2760%2892%2990037-V&partnerID=40&md5=478443aa5a09c6263e17df8d13ff9f20 |
| Is Public | Yes |
| Language Text | English |
| Keyword | VANADIUM; FREE RADICAL; LIPID PEROXIDATION; MICROSOME; LINOLEIC ACID; (HUMAN PLACENTA) |
| Relationship(s) |
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