Determination of 16 phthalate metabolites in urine using automated sample preparation and on-line preconcentration/high-performance liquid chromatography/tandem mass spectrometry
Kato, K; Silva, MJ; Needham, LL; Calafat, AM
HERO ID
789403
Reference Type
Journal Article
Year
2005
Language
English
PMID
| HERO ID | 789403 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Determination of 16 phthalate metabolites in urine using automated sample preparation and on-line preconcentration/high-performance liquid chromatography/tandem mass spectrometry |
| Authors | Kato, K; Silva, MJ; Needham, LL; Calafat, AM |
| Journal | Analytical Chemistry |
| Volume | 77 |
| Issue | 9 |
| Page Numbers | 2985-2991 |
| Abstract | We developed an on-line solid-phase extraction (SPE) method, coupled with isotope dilution high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) and with automated sample preparation, to simultaneously quantify 16 phthalate metabolites in human urine. The method requires a silica-based monolithic column for the initial preconcentration of the phthalate metabolites from the urine and a silica-based conventional analytical column for the chromatographic separation of the analytes of interest. It uses small amounts of urine (100 microL), is sensitive (limits of detection range from 0.11 to 0.90 ng/mL), accurate (spiked recoveries are approximately 100%), and precise (the inter- and intraday coefficients of variation are <10%). The method is not labor intensive, and, because pretreatment of the urine samples was performed automatically using an HPLC autosampler, involves minimal sample handling, thus minimizing exposure to hazardous chemicals. The method was validated on spiked, pooled urine samples and on urine samples from 43 adults with no known exposure to phthalates. The high sensitivity and high throughput (HPLC run time, including the preconcentration step, is 27 min) of this analytical method combined with the ease of use and effective automated sample preparation procedure make it suitable for large epidemiological studies to evaluate the prevalence of human exposure to phthalates. |
| Doi | 10.1021/ac0481248 |
| Pmid | 15859620 |
| Wosid | WOS:000228877500052 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000228877500052 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Adult; Chromatography, High Pressure Liquid/ methods; Humans; Phthalic Acids/ urine; Reproducibility of Results; Sensitivity and Specificity; Solid Phase Extraction/ methods; Tandem Mass Spectrometry/ methods; Urinalysis/methods |
| Is Qa | No |