Measurement of methyl tert-butyl ether and tert-butyl alcohol in human blood by purge-and-trap gas chromatography-mass spectrometry using an isotope-dilution method
Bonin, MA; Ashley, DL; Cardinali, FL; Mccraw, JM; Wooten, JV
| HERO ID | 85709 |
|---|---|
| In Press | No |
| Year | 1995 |
| Title | Measurement of methyl tert-butyl ether and tert-butyl alcohol in human blood by purge-and-trap gas chromatography-mass spectrometry using an isotope-dilution method |
| Authors | Bonin, MA; Ashley, DL; Cardinali, FL; Mccraw, JM; Wooten, JV |
| Journal | Journal of Analytical Toxicology |
| Volume | 19 |
| Issue | 3 |
| Page Numbers | 19:187-191 |
| Abstract | We developed an isotope-dilution method for measuring methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) in whole human blood using a purge-and-trap gas chromatographic-mass spectrometric method. The labeled analogues for MTBE and TBA were [2H12]methyl tert-butyl ether and [2H9]-tert-butyl alcohol, respectively. Volatiles were removed from the blood by direct helium purging of the liquid; were trapped on a Tenax trap; and were desorbed, cryofocused, and chromatographed on a DB-624 capillary column that was connected directly to the ion source of a mass spectrometer. Detection was by mass analysis using a double-focusing magnetic-sector mass spectrometer operating in the full-scan mode at the medium mass resolution of 3000. For the isotope-dilution method, the minimum detection limits in blood (5-10 mL) are 0.01 microgram/L for MTBE and 0.06 microgram/L for TBA. The isotope-dilution method proved to be a big improvement in recovery, reproducibility, and sensitivity over our previous analytical method, which used the labeled ketone, [4-2H3]-2-butanone, as the internal standard for both MTBE and TBA. The isotope-dilution method has sufficient sensitivity for monitoring blood levels of MTBE and TBA in populations exposed to oxygenated fuels containing MTBE. |
| Pmid | 7564298 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | J. Anal. Toxicol. 19:187-191. |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |