Modified phosphotriester method for chemical synthesis of ribooligonucleotides. Part I. Synthesis of riboundecaadenylate and two fragments constituting the sequence of R-17 translation control signal
Sung, W; Narang, S
| HERO ID | 857429 |
|---|---|
| In Press | No |
| Year | 1982 |
| Title | Modified phosphotriester method for chemical synthesis of ribooligonucleotides. Part I. Synthesis of riboundecaadenylate and two fragments constituting the sequence of R-17 translation control signal |
| Authors | Sung, W; Narang, S |
| Journal | Canadian Journal of Chemistry |
| Volume | 60 |
| Page Numbers | 111 |
| Abstract | A modified phosphotriester method has been successfully applied for the chemical synthesis of ribooligonucleotides. The starting material is a fully protected ribomononucleoside containing a 3-phosphotriester group 5. The coupling reaction is performed using mesitylenesulfonyl tetrazole and purification of the product achieved using reversed phase column chromatography. The effectiveness of this method has been demonstrated by achieving an efficient and rapid synthesis of r-A7, r-A11, r5-AAACAUGAGGA-3, and r5-UUACCCAUGU-3 (R-17, translation control sequence).On a appliqu avec succs une mthode de phosphotriester modifi la synthse chimique des ribooligonuclotides. On utilise comme produit de dpart un ribonucloside entirement protg contenant un groupe phosphotriester en position 3(5). On a ralis le couplage en utilisant le msitylne sulfonyle ttrazole et on a purifi le produit par chromatographie sur colonne en phase inverse. On dmontre l'efficacit de cette mthode en ralisant une synthse rapide et efficace du r-A7, r-A11, r5-AAACAUGAGGA-3 et du r5-UUACCCAUGU-3 (R-17, unit de contrle de translation). [Traduit par le journal] |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Is Qa | No |