Inhalation toxicity of gasoline and fuel oxygenates: micronucleus and sister chromatid exchange tests
Schreiner, C; Hoffman, G; Mason, C; Gudi, R
HERO ID
91113
Reference Type
Meetings & Symposia
Subtype
Poster
Year
2004
Language
English
| HERO ID | 91113 |
|---|---|
| Year | 2004 |
| Title | Inhalation toxicity of gasoline and fuel oxygenates: micronucleus and sister chromatid exchange tests |
| Authors | Schreiner, C; Hoffman, G; Mason, C; Gudi, R |
| Abstract | In compliance with the Clean Air Act Section 211(b) for fuel and fuel additive registration, the petroleum industry has conducted a series of comparative multi-endpoint studies of evaporative fractions of seven vapor condensates of gasoline (GVC), or gasoline+ether [methyl t- butyl ether (G/MTBE), ethyl t-butyl ether (G/ETBE), t-amyl methyl ether (G/TAME), diisopropyl ether (G/DIPE)], or alcohol [ethanol (G/EtOH), t-butyl (G/TBA)] oxygenates. Here we present results from micronucleus (MN) and sister chromatid exchange (SCE) tests. Sprague- Dawley rats (5/sex/group used for both studies) were exposed to target concentrations of 0, 2000, 10000, or 20000mg/m3 of each test material, 6 hr/day, 5d/wk for 4 weeks. Positive control groups (5 rats/sex/group) were used for each study; rats were given cyclophosphamide IP, 24hr prior to sacrifice at doses of 5mg/kg for the SCE study and 40mg/kg for the MN study. At treatment end, blood was collected from the abdominal aorta for the SCE test [cultures established within 24 hr of collection], and femurs removed for the MN test. Femoral bone marrow was prepared and stained using a modified Feulgen method. Approx. 21hr after initiation of culture, cells were exposed to 5ug/ml bromodeoxyuridine (BrdU); colcemid was given at 68hr of culture. Cells were harvested after 72hr in culture and processed for SCE evaluation. No significant increased incidence of micronuclei in polychromatic erythrocytes was observed for any test material. Statistically significant increases in SCE over several doses were observed in rats given GVC alone or G/MTBE; females appeared more sensitive than males. G/TAME induced increased SCE in both sexes at the highest dose only. Although several instances of increased SCE were observed in cultured lymphocytes, gasoline and gasoline/oxygenate blends did not induce cytogenetic effects in bone marrow of exposed animals. Results of these studies will be used for comparative risk assessment of gasoline and gasoline/oxygenate blends. |
| Material Type | Poster |
| Dupe Override | No |
| Conference Location | Baltimore, MD, USA |
| Conference Name | Society of Toxicology 43rd Annual Meeting |
| Comments | Inhalation toxicity of gasoline and fuel oxygenates: micronucleus and sister chromatid exchanges tests.Toxicologist 78(1-S): 147. |
| Is Public | Yes |
| Language Text | English |