Liberation of vessel adherent myeloperoxidase by enoxaparin improves endothelial nitric oxide bioavailability

Rudolph, T; Rudolph, V; Klinke, A; Lau, D; Heitzer, T; Meinertz, T; Baldus, S

HERO ID

975871

Reference Type

Journal Article

Subtype

Abstract

Year

2008

Language

English

HERO ID 975871
Material Type Abstract
In Press No
Year 2008
Title Liberation of vessel adherent myeloperoxidase by enoxaparin improves endothelial nitric oxide bioavailability
Authors Rudolph, T; Rudolph, V; Klinke, A; Lau, D; Heitzer, T; Meinertz, T; Baldus, S
Journal Free Radical Biology and Medicine
Volume 45
Issue Suppl.
Page Numbers S135-S135
Abstract Aim: Myeloperoxidase (MPO), a leukocyte-derived heme enzyme binds to the endothelium and depletes vascular nitric oxide (NO) bioavailability in animal models. Unfractionated heparins release vessel-bound MPO and increase endothelial NO bioavailability. Whether low molecular weight heparins also affect circulating MPO levels and NO dependent vasoreactivity however remains elusive. Methods and Results: in a randomized, double-blind, placebo controlled trial patients with stable coronary artery disease received either 1 mg/kg enoxaparin or an equivalent volume of sodium chloride (NaCl) subcutaneously. Enoxaparin led to a significant improvement of FMD (5.51±2.92% vs. 6.55±3.16%, p=0.01) accompanied by a significant increase in plasma MPO levels (2.51 [IR: 2.04-3.62] ng/ml vs. 3.70 [IR: 2.80-5.50] ng/ml; p<0.001) whereas NaCl revealed neither a change in FMD (5.56±3.62% vs. 5.34±3.32%, p=ns) nor in plasma MPO levels (3.04 [IR: 2.22-4.67] ng/ml vs. 2.90 [IR:1.95-4.32] ng/ml; p=ns). The extent of enoxaparin-induced MPO release and the improvement in endothelial function correlated (r=0.67, P<0.001). Conclusion: This study confirms the concept that heparins enhance vascular NO bioavailability by mobilizing vessel bound MPO. These data not only support the notion of extracoagulant, anti-inflammatory properties of heparins but reinforce the concept of MPO-dependent NO oxidation as a central mechanism for regulation of vascular tone in inflammatory vascular disease.
Wosid WOS:000260867900380
Url http://www.sciencedirect.com/science/article/pii/S089158490800631X
Is Certified Translation No
Dupe Override No
Conference Location Indianapolis, IN
Conference Name Society for Free Radical Biology and Medicine 15th Annual Meeting
Conference Date November 19-23, 2008
Is Public Yes
Language Text English
Relationship(s)
  • Is part of a larger document: 3452652