Nitric oxide does escape from red blood cell - Experimental evidence
Piknova, B; Schechter, AN
HERO ID
975877
Reference Type
Journal Article
Subtype
Abstract
Year
2008
Language
English
| HERO ID | 975877 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 2008 |
| Title | Nitric oxide does escape from red blood cell - Experimental evidence |
| Authors | Piknova, B; Schechter, AN |
| Journal | Free Radical Biology and Medicine |
| Volume | 45 |
| Issue | Suppl. |
| Page Numbers | S116-S116 |
| Abstract | Nitric oxide (NO•) regulates a wide array of biological processes and its deficit likely contributes to the severity of some diseases. Discovery of nitrite reductase activity of heme-containing proteins, especially case of deoxyHb, opened the path to the speculations about red blood cell (RBC) as the possible alternative source of NO•, in addition to “classical” L-arginine/NOS pathway. in clinical studies nitrite infusions have led to vasodilatation, and nitrite infusion based therapies to correct NO• deficits are currently under consideration. However, oxyHb also rapidly reacts with NO• and nitrite, and the final products of both oxidative reaction chains are nitrate and metHb. As oxyHb is the prevailing hemoglobin species in blood, main question then is if any of NO• from deoxyHb/nitrite reaction in RBC can escape from the RBC into plasma compartment. So far, this possibility was evaluated mainly in theoretical plane and the general prevailing opinion is that RBC would act as a sink/black hole for NO•. We experimentally tested the hypothesis that NO• produced by nitrite reductase activity of deoxyHb can be liberated from RBC. Measurements were done for different hematocrits and different hemoglobin oxygen saturations. We demonstrate that, despite the large excess of oxyHb, small but measurable quantities of free NO• (in pM /subpM range) are liberated into the surrounding solution at all oxygen saturations and total amount of measured NO• inversely correlates with oxygen saturation. We consider our results to be a direct evidence that nitrite causes vasodilatation directly through nitrite reduction to NO• via deoxyHb and not by some other RBC-related mechanisms. |
| Wosid | WOS:000260867900332 |
| Url | http://www.sciencedirect.com/science/article/pii/S089158490800628X |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Indianapolis, IN |
| Conference Name | Society for Free Radical Biology and Medicine 15th Annual Meeting |
| Conference Date | November 19-23, 2008 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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