Production of hexanoic acid from D-galactitol by a newly isolated Clostridium sp. BS-1
Jeon, BS; Kim, BC; Um, Y; Sang, BI
HERO ID
1454054
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 1454054 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Production of hexanoic acid from D-galactitol by a newly isolated Clostridium sp. BS-1 |
| Authors | Jeon, BS; Kim, BC; Um, Y; Sang, BI |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 88 |
| Issue | 5 |
| Page Numbers | 1161-1167 |
| Abstract | In a study screening anaerobic microbes utilizing D: -galactitol as a fermentable carbon source, four bacterial strains were isolated from an enrichment culture producing H₂, ethanol, butanol, acetic acid, butyric acid, and hexanoic acid. Among these isolates, strain BS-1 produced hexanoic acid as a major metabolic product of anaerobic fermentation with D: -galactitol. Strain BS-1 belonged to the genus Clostridium based on phylogenetic analysis using 16S rRNA gene sequences, and the most closely related strain was Clostridium sporosphaeroides DSM 1294(T), with 94.4% 16S rRNA gene similarity. In batch cultures, Clostridium sp. BS-1 produced 550 ± 31 mL L⁻¹ of H₂, 0.36 ± 0.01 g L⁻¹ of acetic acid, 0.44 ± 0.01 g L⁻¹ of butyric acid, and 0.98 ± 0.03 g L⁻¹ of hexanoic acid in a 4-day cultivation. The production of hexanoic acid increased to 1.22 and 1.73 g L⁻¹ with the addition of 1.5 g L⁻¹ of sodium acetate and 100 mM 2-(N-morpholino)ethanesulfonic acid (MES), respectively. Especially when 1.5 g L⁻¹ of sodium acetate and 100 mM MES were added simultaneously, the production of hexanoic acid increased up to 2.99 g L⁻¹. Without adding sodium acetate, 2.75 g L⁻¹ of hexanoic acid production from D-galactitol was achieved using a coculture of Clostridium sp. BS-1 and one of the isolates, Clostridium sp. BS-7, in the presence of 100 mM MES. In addition, volatile fatty acid (VFA) production by Clostridium sp. BS-1 from D-galactitol and D: -glucose was enhanced when a more reduced culture redox potential (CRP) was applied via addition of Na₂S·9H₂O. |
| Doi | 10.1007/s00253-010-2827-5 |
| Pmid | 20721546 |
| Wosid | WOS:000283095500013 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |