Stimulus-secretion coupling of hypotonicity-induced insulin release in BRIN-BD11 cells
Beauwens, R; Best, Len; Markadieu, N; Crutzen, R; Louchami, K; Brown, P; Yates, AP; Malaisse, WJ; Sener, A
HERO ID
2791674
Reference Type
Journal Article
Year
2006
Language
English
PMID
| HERO ID | 2791674 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Stimulus-secretion coupling of hypotonicity-induced insulin release in BRIN-BD11 cells |
| Authors | Beauwens, R; Best, Len; Markadieu, N; Crutzen, R; Louchami, K; Brown, P; Yates, AP; Malaisse, WJ; Sener, A |
| Journal | Endocrine |
| Volume | 30 |
| Issue | 3 |
| Page Numbers | 353-363 |
| Abstract | The stimulus-secretion coupling for hypotonicity-induced insulin release was investigated in BRIN-BD11 cells. A 50 mM decrease in extracellular NaCl caused a twofold increase in insulin release. The release of insulin evoked by hypotonicity progressively decreased in an exponential manner. The response to extracellular hypotonicity displayed a threshold value close to 20 mOsmol/L and a maximal response at about 70 mOsmol/ L. Hypotonicity also caused a rapid increase in cell volume followed by a regulatory volume decrease (RVD), cell membrane depolarization with induction of spike activity, and a rise in cytosolic Ca2+ concentration. 5-Nitro-2-(3-phenylpropylamino)benzoate inhibited the secretory response to hypoosmolarity, failed to affect the early increase in cell volume but prevented the RVD, and suppressed the hypotonicity-induced plasma membrane depolarization. Insulin release provoked by hypotonicity was inhibited by verapamil, absence of Ca2+, thapsigargin, furosemide, tributyltin, and diazoxide. On the contrary, tolbutamide augmented modestly insulin release recorded in the hypoosmolar medium. Last, a rise in extracellular K+ concentration, while augmenting basal insulin output, failed to affect insulin release in the hypoosmolar medium. Thus, the insulin secretory response to hypotonicity apparently represents a Ca2+-dependent process triggered by the gating of volume-sensitive anion channels with subsequent depolarization and gating of voltage-sensitive Ca2+ channels. |
| Doi | 10.1007/s12020-006-0014-3 |
| Pmid | 17526948 |
| Wosid | WOS:000246018300014 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | hypotonicity; insulin release; BRIN-BD11 cells |