[The kinetic parameters of retainment and release of deposited in human tissue structures calcium]
Ermakova, IP; Potanina, TV; Pronchenko, IA; Larina, IM; Sevast'ianov, VI
HERO ID
4944895
Reference Type
Journal Article
Year
2014
Language
Russian
PMID
| HERO ID | 4944895 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | [The kinetic parameters of retainment and release of deposited in human tissue structures calcium] |
| Authors | Ermakova, IP; Potanina, TV; Pronchenko, IA; Larina, IM; Sevast'ianov, VI |
| Journal | Rossiĭskii Fiziologicheskiĭ Zhurnal Imeni I.M. Sechenova |
| Volume | 100 |
| Issue | 11 |
| Page Numbers | 1335-1345 |
| Abstract | The analysis of retainment and release kinetics of deposited in tissue structures calcium was made in the hypercalcemic conditions in 28 healthy volunteers (22 males and 6 females) of the age of 33 ± 6.5 years via drip infusion (Groups 1, 2) and in 9 individuals (3 males and 6 females) in 12 trials via stream infusion (Group 3). By the end of each hour after the termination of calcium infusion the amount of calcium retained in tissues was calculated (Mtis./kg); the parameters of its binding (specific buffer volume--β3 sp, association constant--Ka, number of binding centers--n) were established using the Langmuir and Scetchard coordinates. The Group 1 volunteers (n = = 12) showed a section of positive cooperativity (a direct regression on Sketchard coordinates, Hill coefficient 3.36 ± 1.63) and 2 sections of the consecutive calcium separation from one set of noninteracting centers. 5 volunteers of Group 2 and 8 volunteers of Group 3 demonstrated a slight calcium delivery to tissues after 1 hour of observation which then followed for 2 volunteers of Group 2 and for 2 volunteers of Group 3. Other volunteers of Groups 2 and 3 showed a release of tissue-deposited calcium via the mechanism of the consecutive separation from one set of noninteracting centers with βsp 3 times less and Ka 7 times higher than with the calcium infusion. The excretion of calcium in urine was the highest in Group 1 and the lowest in Group 3. The [Ca2+] and Mtis./kg values were normalized in Groups 1 and 2 the next morning and in Group 3 after 2-3 hours of observation. An assumption was made about the relationship between the tissue and kidney [Ca2+] normalizing mechanisms with hypercalcemia. |
| Pmid | 25665412 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | Russian |