Toxicity and teratogenicity of food additive chemicals in the developing chicken embryo
Verrett, MJ; Scott, WF; Reynaldo, EF; Alterman, EK; Thomas, CA
HERO ID
689851
Reference Type
Journal Article
Year
1980
Language
English
PMID
| HERO ID | 689851 |
|---|---|
| In Press | No |
| Year | 1980 |
| Title | Toxicity and teratogenicity of food additive chemicals in the developing chicken embryo |
| Authors | Verrett, MJ; Scott, WF; Reynaldo, EF; Alterman, EK; Thomas, CA |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 56 |
| Issue | 2 |
| Page Numbers | 265-273 |
| Abstract | HEEP COPYRIGHT: BIOL ABS. Toxicity of 80 chemicals which have food additive use were investigated by administration to developing chicken embryos. Test conditions (4) were used: injection via air cell and via yolk; and at 2 times, preincubation (0 h) and 96 h. For each condition, at least 100 embryos/dose level were treated at a minimum of 5 dose levels. Appropriate groups of vehicle controls and untreated controls were included in all experiments. LD50 values were determined for each test condition. All embryos and hatched chicks were examined grossly for abnormalities in development, both functional and structural. All abnormalities were tabulated and compared with values for vehicle controls to determine teratogenicity. Organic and inorganic chemicals were tested, including gums and flavoring agents. Levels tested ranged from 0.50-75 mg/egg; the maximum volume injected with 100 mul. In some cases, test levels were low because of inability to find a nontoxic solvent for test chemical. No toxic compounds were found among those tested. A range of LD50 values was found. Investigation of teratogenicity of these chemicals revealed that 10 of 80 produced significant numbers of abnormal birds under 1 or more test conditions. Chicken embryo test can demonstrate the teratogenic potential of compounds. It is selective and does not respond nonspecifically to any agent introduced during development. The test may be useful for screening large numbers of compounds to identify those that should be subjected to more rigorous evaluation. |
| Pmid | 7466825 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: TOXICOL APPL PHARMACOL 56 ISSN: |
| Is Public | Yes |
| Language Text | English |
| Keyword | Animals; Chick Embryo; Food Additives/administration & dosage/*toxicity; Lethal Dose 50; *Teratogens |
| Is Qa | No |
| Relationship(s) |
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