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HERO ID
1003602
Reference Type
Journal Article
Title
Oxidation of arsenite by two β-proteobacteria isolated from soil
Author(s)
Bachate, SP; Khapare, RM; Kodam, KM
Year
2012
Is Peer Reviewed?
Yes
Journal
Applied Microbiology and Biotechnology
ISSN:
0175-7598
EISSN:
1432-0614
Volume
93
Issue
5
Page Numbers
2135-2145
Language
English
PMID
21983709
DOI
10.1007/s00253-011-3606-7
Web of Science Id
WOS:000300657900031
Abstract
Two heterotrophic As(III)-oxidizing bacteria, SPB-24 and SPB-31 were isolated from garden soil. Based on 16S rRNA gene sequence analysis, strain SPB-24 was closely related to genus Bordetella, and strain SPB-31 was most closely related to genus Achromobacter. Both strains exhibited high As(III) (15 mM for SPB-24 and 40 mM for SPB-31) and As(V) (>300 mM for both strains) resistance. Both strains oxidized 5 mM As(III) in minimal medium with oxidation rate of 554 and 558 μM h(-1) for SPB-24 and SPB-31, respectively. Washed cells of both strains oxidized As(III) over broad pH and temperature range with optimum pH 6 and temperature 42°C for both strains. The As(III) oxidation kinetic by washed cells showed K (m) and V (max) values of 41.7 μM and 1,166 μM h(-1) for SPB-24, 52 μM and 1,186 μM h(-1) for SPB-31. In the presence of minimal amount of carbon source, the strains showed high As(III) oxidation rate and high specific arsenite oxidase activity. The ability of strains to resist high concentration of arsenic and oxidize As(III) with highest rates reported so far makes them potential candidates for bioremediation of arsenic-contaminated environment.
Keywords
Arsenite oxidation; Achromobacter; Bordetella
Tags
IRIS
•
Arsenic Hazard ID
1. Initial Lit Search
PubMed
WOS
ToxNet
WOS
Considered New
2. Lit Search Updates through Oct 2015
WOS
Considered
4. Considered through Oct 2015
6. Cluster Filter through Oct 2015
7. Other Studies through Oct 2015
Other
•
Arsenic (Inorganic)
1. Literature
PubMed
Toxline, TSCATS, & DART
Web of Science
Lit search updates through Oct 2015
3. Hazard ID Screening
Other potentially supporting studies
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