US EPA

1005476 

Journal Article 

Abstract 

Enhancement of dinoseb-induced teratogenicity by maternal food deprivation in mice 

Preache, M; Gibson, JE 

1974 

1 

Toxicology and Applied Pharmacology
ISSN: 0041-008X
EISSN: 1096-0333 

29 

1 

122 

English 

is part of a larger document 3378179 Abstracts of papers for the Thirteenth Annual Meeting of the Society of Toxicology, Washington, D.C. March 10–14, 1974

Dinoseb (2-sec-butyl-4,6-dinitrophenol), a commonly used herbicide, has teratogenic potential in mice at or near the dose levels required for maternal toxicity (Gibson, Fd. Cosmet. Toxicol.11, 31, 1973). Food deprivation was combined with dinoseb treatment to determine whether the teratogenicity would be altered. Pregnant mice were assigned to 1 of 4 groups that were either maintained on normal diet and treated with dinoseb (15.8 mg/kg/day, ip)or given a control injection of the vehicle on days 10-12, or were deprived of food but not water for 24 hr on the ninth day and given the dinoseb (15.8 mg/kg/day, ip) or control vehicle injections on the succeeding 3 days. The fetuses were removed by cesarean section on day 19 and were weighed, measured (crown-rump distance), and examined for external abnormalities. Mothers that received control injections, whether or not they were food deprived, produced litters in which greater than 95% of the fetuses were normal. Fetal weight and the proportion of normal fetuses/litter were decreased by dinoseb alone or in combination with food deprivation. Litters from dinoseb-treated mothers that had been food deprived had a higher incidence of anomalies than those of nondeprived mothers, but fetal weight was not further reduced by the deprivation. External anomalies included club feet, a reduction in the size of the fore limbs and the tail and absence of digits on both fore and hind limbs. Skeletal examinations indicated that the club foot anomaly was related to a reduction in size or absence of the tibia and also revealed a high incidence of fused ribs and vertebrae as well as the absence of digital bones. Soft tissue examinations most frequently revealed hydronephrosis sometimes accompanied by hydroureter. In conclusion, maternal food deprivation for 24 hr preceding dinoseb treatment altered dinoseb-induced teratogenicity by increasing the incidence of anomalies that were qualitatively the same as those observed with dinoseb alone. 

Thirteenth Annual Meeting of the Society of Toxicology 

Washington, DC 

March 10-14, 1974