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1009428 
Journal Article 
Immobilization of glucoamylase onto alternative acrylonitrile-butadiene copolymer by amidination reaction 
Yajima, H; Hirose, A; Ishii, T; Ohsawa, T; Endo, R 
1989 
Yes 
Biotechnology and Bioengineering
ISSN: 0006-3592
EISSN: 1097-0290 
33 
6 
795-798 
English 
18587983 
WOS:A1989R951600018 
Numerous methods have been so far reported for the immobilization of amylases using specific kinds of fixation techniques and supports. Handa et al. have studied the preparation and properties of these enzymes immobilized onto partially imidoesterized polyacrylonitrile (PAN) as the support using an amidination reaction. Glucoamylase (GA) immobilized onto PAN showed a high durability against repeated use and a slight decrease in enzymic activity was observed after reusing it 10 times for the enzyme reaction. This communication is concerned with the preparation and properties of immobilized GA covalently attached to alternative acrylonitrile-butadiene (AB) copolymer. 
glucan 1,4- alpha -glucosidase; immobilization; copolymers; supports; reaction; acrylonitrile; amidination; butadiene; Biotechnology and Bioengineering Abstracts; W 30220:Enzyme