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1021923 
Journal Article 
Mycobased biosynthesis of silver nanoparticles and studies of its synergistic
antibacterial activity combined with cefazolin antibiotic against selected organisms 
Singh, P; Kumar, R; Raja, RB; Kalaichelvan, PT 
2011 
Yes 
Australian Journal of Basic and Applied Sciences
ISSN: 1991-8178 
5 
8 
1412-1427 
Silver nanoparticles were synthesized biologically from non pathogenic fungus and its synergistic antibacterial activity with the antibiotic cefazolin was studied in detail. The nanoparticles were characterized using UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). In the UV - Vis spectrum the peak at 420nm confirms the synthesis of silver nanoparticles and from TEM studies silver nanoparticles with different shapes and sizes were observed. The size of the silver nanoparticles was observed in the range of 30-50 nm with spherical shape. The synthesis of nanoparticles was rapid, stable for several months and involves purely 'green synthesis' which does not involve any toxic chemicals and by products. The antibacterial activity for silver nanoparticles, antibiotic and antibiotic conjugated with silver nanoparticles was assessed using the standard broth dilution method and pour plate technique for determining the minimum inhibitory concentration (MIC). Organisms used for this study are Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive). The antibacterial effect of the antibiotic and the silver nanoparticles was found separately and compared with that of combined effect of antibiotic and silver nanoparticles and the combination of antibiotic with silver nanoparticles was found to be very effective compared to its individual effect. The current study opens light to a novel drug with broad spectrum antibacterial activity. [ABSTRACT FROM AUTHOR] ght of Australian Journal of Basic & Applied Sciences is the property of INSInet Publications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) 
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