US EPA

1024773 

Journal Article 

Abstract 

Effect of chelating agent (DMSA) and Vitamin C on nickel induced testicular toxicity in rats: toxicological, biochemical and histological study 

Laila, AM; Azza, FA; Wafaa, EM; Tarek, AA; Amany, OS; Abir, SA 

2003 

1 

Toxicology
ISSN: 0300-483X
EISSN: 1879-3185 

DART/TER/4000258 

191 

1 

52 

English 

WOS:000185544700158 

The aim of the present work was to study the effect of nickel toxicity induced by prolonged exposure to nickel chloride on testes of albino rats, as well as to investigate the effect of a chelating agent (DMSA) and antioxidant (Vitamin C) on such toxicity. The results of the present study showed that subcutaneous administration of NiCl2 (4.4 mg/kg per day) for 5 weeks resulted in a significant increase of nickel and malondialdehyde (MDA) levels and a significant decrease of reduced glutathione (GSH) and superoxide dismutase (SOD) activity in the testicular tissues. Histological examination of testes showed multiple degenerative changes and malformation of spermatozoa. The present study revealed that when Vitamin C was administered concomitantly with NiCl2 for 5 weeks, it caused a significant effect in preserving the histological and ultrastructural picture of the testis and more or less normalization of the antioxidant defense system. It also caused a lowering of nickel levels (but not reaching the control level). On the other hand, when DMSA was administered for 5 days after administration of NiCl2 for 5 weeks, this caused a significant lowering of the nickel level which reached control values, with a mild improvement of the antioxidant defense system and a lesser improvement of the histological and ultrastructural affects. 

Rats; Animals; Male; Nickel/TOXICITY; Succimer/PHARMACOLOGY; Ascorbic Acid/PHARMACOLOGY; Chelating Agents/PHARMACOLOGY; Testis/DRUG EFFECTS/METABOLISM/ULTRASTRUCTURE; Superoxide Dismutase/METABOLISM; Glutathione/METABOLISM; 7440-02-0; 7718-54-9; 304-55-2; 50-81-7; NO CAS RN; 70-18-8