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1038723 
Journal Article 
An efficient method for isolation of plasmid DNA from methylotrophic bacteria 
Brenner, V; Holubová, I; Hubácek, J 
1990 
Yes 
Folia Microbiologica
ISSN: 0015-5632
EISSN: 1874-9356 
35 
454-455 
English 
A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.