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HERO ID
1038723
Reference Type
Journal Article
Title
An efficient method for isolation of plasmid DNA from methylotrophic bacteria
Author(s)
Brenner, V; Holubová, I; Hubácek, J
Year
1990
Is Peer Reviewed?
Yes
Journal
Folia Microbiologica
ISSN:
0015-5632
EISSN:
1874-9356
Volume
35
Issue
5
Page Numbers
454-455
Language
English
PMID
2125292
Abstract
A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.
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