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1039 
Journal Article 
Abstract 
Combined chronic feeding and three-generation reproduction study of sodium saccharin in the rat 
Taylor; JM; Friedman, L 
1974 
1 
Toxicology and Applied Pharmacology
ISSN: 0041-008X
EISSN: 1096-0333 
29 
1 
154 
English 
is part of a larger document 3378179 Abstracts of papers for the Thirteenth Annual Meeting of the Society of Toxicology, Washington, D.C. March 10–14, 1974
The carcinogenic and reproductive effects of sodium saccharin were studied in the Charles River CD rat in a combined chronic and 3-generation reproduction study. For the chronic study, 48 male and 48 female F1a weanling offspring per test group were selected and continued on the same test regimen as their parents. The dietary levels were 0, 0.01, 0.1, 1.0, 5.0 and 7.5% sodium saccharin. Calcium cyclamate was fed as a reference compound at 5.0%. The rats were mated to produce 2 litters. Serial sacrifices were performed at 14 and 18 months. The study was continued until the number of survivors in a group fell to 20% of the starting number. The last rats were killed approximately 28 months after the first weanlings were selected for the chronic study. There were no significant differences between test and control groups in hematological values, organ weights or survival. Average weaning weights were decreased in litters from parents receiving 5 and 7.5% sodium saccharin and 5% calcium cyclamate; other reproductive indices showed scattered variations but these were not consistent for all generations. During the chronic study some of the initial weaning weight depression was overcome, but rats on 5 and 7.5% sodium saccharin and 5% calcium cydamate had lower average body weights throughout the study. Histopathological examination of the urinary bladders from rats on the chronic study longer than 18 months revealed an increased incidence of transitional cell carcinomas in the 7.5% sodium saccharin group. 
Thirteenth Annual Meeting of the Society of Toxicology 
Washington, D.C. 
March 10–14, 1974