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1069315 
Journal Article 
Hypomethylation and histological changes induced by chloroform administered by oral gavages in corn oil to mice 
Mostafaw, AAZM; Zahran, FM; Guirgis, A; Zein, N 
2009 
3 
3 
1569-1576 
5-Methylcytosine (5-Mec) is a natural modification of DNA methyltransferase (DNA MTase), S-adenosyl- methionine (SAM) uses as the Me donor and yields S-adenosyl homocysteine (SAH). The effects of chloroform on DNA methylation and histopathol. alterations occurs in the liver were studied. Male and female Swiss albino mice were treated by chloroform through oral gavage. Different concns. 130, 238, 277 and 477mg chloroform/kg mice dissolved in corn oil (10mL/kg mice), for 19, 33 and 54 days. Whereas, treatment with chloroform with high concn. for the longest period led to progressive hypomethylation of liver DNA as detd. by gel electrophoresis to HpaII and MspI digested DNA and likewise. Moreover, light microscope appeared malignant cells with polymorphic nuclei, and sever displasia appeared with necrosis in moderate dose. ALT and albumin serum levels were also measured as a biomarker for liver dysfunction where ALT level showed significant elevation and albumin level showed significant depression. Our data concluded that high doses of chloroform produce regenerative cell proliferation, and hypomethylation of DNA which are a common feature of non-genotoxic carcinogens. Elevation of ALT level and decrease of albumin level confirmed liver disorder using high doses of chloroform.