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1254011 
Journal Article 
LC-MS compatible HPLC separation of xenobiotics and their phase I and phase II metabolites: Simultaneous anion exchange and reversed-phase chromatography 
Johnston, JJ; Draper, WM; Stephens, RD 
1991 
Yes 
Journal of Chromatographic Science
ISSN: 0021-9665 
BIOSIS/92/06336 
29 
12 
511-516 
English 
BIOSIS COPYRIGHT: BIOL ABS. Benzene and five of its mammalian metabolites, phenol, phenyl-beta-D-glucuronide, phenylsulfate, phenylmercapturate, and t,t-muconic acid, are separated on a methylstyrene-divinylbenzene-based anion exchange-reversed-phase column. The retention of the model compounds is manipulated by modifying the type, ionic strength, and pH of the mobile phase buffer, and the type and percent of organic mobile phase modifier. The separations developed are compatible with both particle beam (PM) and thermospra (TSP) interfaces used in liquid chromatography-mass spectrometry (LC-MS). These separations should be adaptable to the study of a wide range of xenobiotics and their phase I and phase II metabolites, and provide an LC-MS compatible alternative to the use of ion pairing reagents. 
Biochemical Methods-General; Biochemical Studies-General; Biophysics-General Biophysical Techniques; Biophysics-Molecular Properties and Macromolecules; Metabolism-General Metabolism; Toxicology-General; Neoplasms and Neoplastic Agents-Carcinogens and Carcinogenesis