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1293962 
Journal Article 
Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance ((1)H NMR) based metabolomics 
Zhang, L; Liu, X; You, L; Zhou, D; Wang, Q; Li, F; Cong, M; Li, L; Zhao, J; Liu, D; Yu, J; Wu, H 
2011 
Yes 
Environmental Toxicology and Pharmacology
ISSN: 1382-6689
EISSN: 1872-7077 
32 
2 
218-225 
English 
21843802 
WOS:000294936700014 
Benzo(a)pyrene is an important polycyclic aromatic hydrocarbon (PAH) which causes carcinogenic, teratogenic and mutagenic effects in various species and the level of contamination of this toxic agent in the marine environment is of great concern. In this study, metabolic responses induced by two doses (0.02 and 0.2μM) of BaP were characterized in the gill tissues of Manila clam Ruditapes philippinarum after exposure for 24, 48 and 96h. The high dose (0.2μM) of BaP induced the disturbances in energy metabolism and osmotic regulation based on the metabolic biomarkers such as succinate, alanine, glucose, glycogen, branched chain amino acids, betaine, taurine, homarine, and dimethylamine in clam gills after 24h of exposure. In addition, hormesis induced by BaP was found in clams exposed to both doses of BaP. Overall, our results demonstrated the applicability of metabolomics for the elucidation of toxicological effects of marine environmental contaminants in a selected bioindicator species such as the Manila clam. 
Benzo(a)pyrene; Biomarker; Manila clam; Metabolomics; NMR; Toxicological effects