US EPA

1306981 

Journal Article 

Methylene blue inhibits the stimulation of bone mass by high doses of estradiol in male rats 

Broulik, PD; Schreiber, V 

1994 

Yes 

Endocrine Regulations
ISSN: 1210-0668 

28 

3 

141-144 

English 

7858245 

To elucidate the effect of methylene blue on bone metabolism we examined the rats treated with estradiol, with methylene blue alone and with estradiol and methylene blue. The mineral bone mass in the femurs of the animals was measured quantitatively. A significant increase of bone density and bone mineral content was found in the estradiol treated animals compared to animals without estradiol. The rise in bone mineral content after estradiol was inhibited by methylene blue. Methylene blue alone did not affect bone mineral mass. From our work the possibility arose that beside well known effect of estradiol on osteoblast receptors also other mechanism of estrogen action exists which possibly may include the effect of estrogen on cytosolic guanylate cyclase producing cGMP.