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Citation
Tags
HERO ID
1317960
Reference Type
Journal Article
Title
Significant differences in global genomic DNA methylation by gender and race/ethnicity in peripheral blood
Author(s)
Zhang, FF; Cardarelli, R; Carroll, J; Fulda, KG; Kaur, M; Gonzalez, K; Vishwanatha, JK; Santella, RM; Morabia, A
Year
2011
Is Peer Reviewed?
Yes
Journal
Epigenetics
ISSN:
1559-2294
EISSN:
1559-2308
Publisher
TAYLOR & FRANCIS INC
Location
PHILADELPHIA
Volume
6
Issue
5
Page Numbers
623-629
Language
English
PMID
21739720
Web of Science Id
WOS:000290203600012
Abstract
Reduced levels of global DNA methylation are associated with genomic instability and are independent predictors of cancer risk. Little is known about the environmental determinants of global DNA methylation in peripheral blood. We examined the association between demographic and lifestyle factors and levels of global leukocyte DNA methylation in 161 cancer-free subjects enrolled in the North Texas Healthy Heart Study aged 45-75 years in 2008. We used in-person interviews for demographics and lifestyle factors, a self-administrated Block food frequency questionnaire for diet, and bioelectrical impedance analysis and CT-scan for body composition. We measured genomic DNA methylation using bisulfite conversion of DNA and pyrosequencing for LINE-1. Body composition measures including body mass index, waist circumference, areas of subcutaneous fat and visceral fat, percent of fat mass and fat-free mass were not associated with global genomic DNA methylation after controlling the effect of age, gender and race/ethnicity. Instead, female gender was significantly associated with a reduced level of global methylation (β = -2.77, 95% CI: -4.33, -1.22). Compared to non-Hispanic whites, non-Hispanic blacks (β = -2.02, 95% CI: -3.55, -0.50) had significantly lower levels of global methylation. No association was found with age, cigarette smoking, alcohol drinking and dietary intake of nutrients in one-carbon metabolism. Global leukocyte DNA methylation differs by gender and race/ethnicity, suggesting these variables need to be taken into consideration in studies of global DNA methylation as an epigenetic marker for cancer.
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