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1334768 
Journal Article 
Two-stage exposure of Syrian-hamster-embryo cells to environmental carcinogens: Superinduction of ornithine decarboxylase correlates with increase of morphological-transformation frequency 
Dhalluin, S; Ellas, Z; Cruciani, V; Bessi, H; Poirot, O; Rast, C; Gate, L; Pages, N; Tapiero, H; Vasseur, P; Nguyen-Ba, G 
1998 
Yes 
International Journal of Cancer
ISSN: 0020-7136
EISSN: 1097-0215 
75 
5 
744-749 
English 
9495243 
WOS:000072498100013 
As part of environmental toxicology, it is important to assess both the carcinogenic potential of xenobiotics and their mode of action on target cells. Since dysregulation of ornithine decarboxylase (ODC), a rate-limiting enzyme of polyamine biosynthesis, is considered as an early and essential component in the process of multistage carcinogenesis, we have studied the mode of ODC induction in Syrian-hamster-embryo(SHE) cells stage-exposed to carcinogens and to non-carcinogens. One-stage (5 hr) treatment of SHE cells with 50 muM clofibrate (CLF), a non-genotoxic carcinogen, or with 0.4 muM benzo(a)pyrene (BaP), a genotoxic carcinogen, slightly decreased basal ODC activity. Using the 2-stage exposure, 1 hr to carcinogen, then replacement by TPA for 5 hr, the ODC activity was higher than that obtained with TPA alone. This ODC superinduction was not observed when SHE cells were similarly pre-treated with non-carcinogenic compounds. Several environmental chemicals, pesticide 
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