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1338144 
Journal Article 
A wet-primed extracorporeal membrane oxygenation circuit with hollow-fiber membrane oxygenator maintains adequate function for use during cardiopulmonary resuscitation after 2 weeks on standby 
Karimova, A; Robertson, A; Cross, N; Smith, L; O'Callaghan, M; Tuleu, C; Long, P; Beeton, A; Han, JH; Ridout, D; Goldman, A; Brown, K 
2005 
Yes 
Critical Care Medicine
ISSN: 0090-3493
EISSN: 1530-0293 
33 
7 
1572-1576 
16003064 
WOS:000230630300014 
Objective: To assess the durability of wet-preprimed
extracorporeal membrane oxygenation (ECMO) circuits for potential use in resuscitation after a 2
-wk period of storage. Design: Experimental laboratory study. Setting: Tertiary care pediatric
cardiac intensive care unit. Subjects: None. Interventions and Measurements: 14 ECMO circuits
(polyvinyl chloride and super-Tygon tubing with hollow-fiber oxygenator, Medos Hilite 800LT) were
primed with crystalloid under sterile conditions and stored for 0 (control, n = 4), 7 (n = 5) and
14 (n = 5) days and maintained at 8 degrees C on pump at 10 rpm and gas flow at 0.2 L/min. Daily
samples were inspected for plasticizers by means of high-performance liquid chromatography and
for microorganisms by culture and polymerase chain reaction techniques. After storage, the
oxygenators were primed with red blood cells (hemoglobin, 12 g/dL) and tested in vitro with a
deoxygenator according to Association for Advancement of Medical Instrumentation standards.
Oxygen and CO, transfer rates were calculated by standard formulae at maximum blood flow (800
mL/min) and maximum sweep gas flow (1.6 L/min). Conclusions. A wet-preprimed ECMO circuit with
hollow-fiber membrane oxygenator can be stored for up to 2 wks with adequately preserved function
if prepared appropriately. These data may improve safe access to rapid-response ECMO support. 
infant; extracorporeal membrane oxygenation (ECMO); cardiac arrest; resuscitation; sterility; plasticizer