A short-term repeated administration to low-dose DEHP, BPA, and BADGE associated with the development of type 2 diabetes mellitus in adolescent male Sprague Dawley rats
Kim, S; Yang, Y; Park, M; Ahn, JiH; Hong, Y
Background/Aims:
Some of endocrine disruptors such as Di (2-ethylhexyl) phthalate (DEHP) and bisphenol A (BPA) may be related to the development of diabetes mellitus at higher doses than environmental or occupational exposure level. We investigated that the short-term administration of DEHP, BPA, and BADGE in human exposure ranges effects in vivo on the development of type 2 diabetes mellitus.
Methods:
The study used male Sprague Dawley rats (5 weeks). The experimental group rats were administered DEHP (0.75, 7.5, 15, 150 mg/kg/d), BPA (0.005, 0.05, 0.1 mg/kg/d), and BADGE (0, 0.5, 5, 10 mg/kg/d) during 7 days by a gavage. We measured the body weight, the fasting blood glucose from the tail vein, and insulin, leptin, adiponectin, and T3 in plasma. The Otsuka Long-Evans Tokushima Fatty rats were used as positive control.
Results:
Body weights were not significantly different when compared with the control group. The insulin levels were significantly increased in DEHP 0.75 and 7.5 mg/kg/d. Fasting blood glucose levels in BPA 0.05 and 0.1 mg/kg/d were significantly decreased. The insulin levels were tending to increase all BADGE treatment groups. Fasting blood glucose and T3 levels in BADGE 10 mg/kg/d and leptin and adiponectin in BADGE 0.5 mg/kg/d were significantly increased. In the Otsuka Long-Evans Tokushima Fatty rats, the OGTT, adiponectin, T4, TSH, and MDA were changed.
Conclusion:
These results suggest that short-term low-dose exposure to DEHP, BPA, and BADGE could be associated with the developing type 2 diabetes mellitus.