US EPA

1343582 

Journal Article 

Use of the anion-exchange resin amberlite IRA-93 for the separation of arsenite and arsenate in aqueous samples 

Bissen, M; Gremm, T; Koklu, U; Frimmel, FH 

2000 

Yes 

Acta Hydrochimica et Hydrobiologica
ISSN: 0323-4320
EISSN: 1521-401X 

28 

1 

41-46 

WOS:000086344300006 

The method described uses the separation of As(III) and As
(V) species in aqueous samples by means of the anion-exchange resin Amberlite IRA-93. The samples
were acidified using acetic acid and passed through a glass column filled with pre-treated
Amberlite IRA-93 resin. As(III) was poorly adsorbed on the anionic exchanger material, whereas
As(V) was retained. The arsenic concentration was measured in the column effluent by graphite
furnace AAS (GF-AAS). The retained As(V) was eluted from the column using 1 M NaOH. Prior to the
determination of the As(V) concentration in the NaOH eluate, the eluate was passed through a
glass column filled with a cation-exchange resin (Amberlite 200) to remove sodium ions and
minimize the Na+ interference with the AAS determination. After calibration the method was
applied to the separation of As(III) and As(V) species in two aqueous extracts of arsenic
contaminated soils. The results were compared with those obtained from an on-line separation and
determination of As(III) and As(V) in the aqueous soil extracts using a state of the art HPLC-
ICP-MS system. 

arsenic; speciation; anion-exchange resin; GF-AAS; HPLC-ICP-MS