Health & Environmental Research Online (HERO)


Print Feedback Export to File
1441295 
Journal Article 
Quantitative gas chromatographic determination of hexachloro-1,3-butadiene in whole rat blood at part per trillion levels 
Kastl, PE; Hermann, EA 
1983 
Yes 
Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 
NIOSH/00161266 
280 
2 
390-393 
English 
6662894 
WOS:A1983RY90800020 
A simple gas chromatographic method was developed for the determination of hexachloro-1,3-butadiene (87683) (HCBD) in whole rat blood. Blood was obtained from male F-344-rats. Blood standards were prepared by adding a known quantity of HCBD to 100 milligrams whole rat blood. To determine the percent of recovery, all blood standards were extracted with hexane (110543) with an internal 1,2,3,4-tetrachlorobenzene (634662) (TCB) standard and then were analyzed by gas chromatography with electron capture detection. Recoveries of HCBD from the whole blood ranged from 85.4 to 122.3 percent, with the mean percent recovery for the entire blood concentration range at 101.0 percent. Coefficients of variation ranged from 0.9 percent at 30.3 nanograms per milliliter (ng/ml) to 11.5 percent at 0.0182ng/ml. No peaks were apparent to interfere with the quantitative analysis of HCBD. The retention time of HCBD was 2.0 minutes and that of TCB was 6.2 minutes. The detector response for both HCBD and TCB was linear for a concentration from 0.017ng/ml to 167.0ng/ml and 0.917ng/ml to 555.0ng/ml, respectively. The authors conclude that the method described is a simple and sensitive way to determine HCBD in whole rat blood in concentrations as low as parts per trillion. The need for colorimetric or ultraviolet spectroscopic detection of an HCBD titration product is avoided. 
DCN-149737; Clinical chemistry; Gas chromatography; Analytical instruments; Laboratory techniques; Chromatographic analysis; Toxicology; Quantitative analysis; Analytical methods; Monitoring systems; Analytical chemistry; Monitors