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1509437 
Technical Report 
Evaluation Of Biomonitoring Systems For Assessment Of Contaminated Waters And Sediments At Us Army Installations. [ADA326455] 
Burton, DT; Herriott, RS; Turley, SD 
1995 
Wye Research Education Center 
Queenstown, MD 
A326455 
1189 
Contaminated groundwater, which contained multiple heavy metals and chlorinated aliphatic hydrocarbons, from the Canal Creek aquifer (well CC-27B) of the US Army Aberdeen Proving Ground-Edgewood Area, Aberdeen, Maryland, was evaluated for toxicity to aquatic organisms. Toxicity was detected at various groundwater concentrations by 6 of 8 biomonitoring systems. The lowest concentration of groundwater that caused no observable adverse effect (NOEC) at pH 4 was 10% ground water by volume in the following assays: 4-d green alga Selenastrum capricornutum) growth est; 7-d cladoceran (Ceriodapnia dubia) survival ana reproduction test; and 96-h frog (Xenopus laevis) embryo teratogenesis assay - Xenopus (FETAX). A NOEC of 18% groundwater volume occurred in 7-d fathead minnow (Pimephales promelas) survival and growth tests. Buffered groundwater (pH 7) was less toxic and/or not toxic in 7-d fathead minnow tests and in FETAX assays. The 10% groundwater by volume NOECs for the green alga and cladoceran at pH 4 did not change when the organisms were exposed to buffered groundwater at pH 7. The Ames assay for mutagenicity was negative in all cases. Sporadic incidences of lesions were found in Japanese medaka(Oryzias latipes) at concentrations up to 25% groundwater by volume after 9 months of exposure. Fish growth was affected by 9 months of exposure; fish were smaller when grown in groundwater diluted with Wear Branch of Canal Creek water. 
adverse conditions; aliphatic hydrocarbons; aquatic organisms; aquifers; army facilities; assaying; biological detection; biomonitoring; buffers; cariodaphnia dubis; chlorinate aliphatic hydrocarbons; chlorinated hydrocarbons; concentration (chemistry); contamination; embryo teratogenesis; embryos; exnopus; ground water; growth (general); heavy metals; maryland; monitors; ozyzias latipes; pimphales promelas; selenastrum capriocornutum; streams; survival (general); teratogenic compounds; test and evaluation; toxicity; water pollution; xenopus laevis