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Citation
Tags
HERO ID
1512890
Reference Type
Journal Article
Title
Hypomyelination in three Weimaraner dogs
Author(s)
Millán, Y; Mascort, J; Blanco, A; Costa, C; Masian, D; Guil-Luna, S; Pumarola, M; Martin de Las Mulas, J
Year
2010
Is Peer Reviewed?
Yes
Journal
Journal of Small Animal Practice
ISSN:
0022-4510
Volume
51
Issue
11
Page Numbers
594-598
Language
English
PMID
20973788
DOI
10.1111/j.1748-5827.2010.00997.x
Web of Science Id
WOS:000283597400006
URL
http://
://WOS:000283597400006
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Abstract
Hypomyelination syndrome of the Weimaraner dog is a disease characterised by a reduction or absence of myelin in the axons of the central nervous system (CNS) exclusively. The objective of this study was to analyse the cause of this deficiency of myelin. Tissue samples of the CNS of three Weimaraner dogs with neurological signs were fixed in 10% formalin and embedded in paraffin wax, and histochemical, immunohistochemical and ultrastructural studies were performed. Histochemical staining with haematoxylin and eosin and Kluver-Barrera techniques showed generalised pallor in the peripheral areas of the ventral and lateral funiculi of the spinal cord. Immunohistochemical analysis showed a weak expression of both proteolipid protein (PLP) and myelin basic protein (MBP) and a marked decrease of Olig2(+) cells in the demyelinated areas. The immunohistochemical findings suggested a myelination or remyelination failure because of the smaller population of oligodendrocytes. However, PLP gene mutations may also be the cause of the decrease of PLP expression as described in other species.
Keywords
pelizaeus-merzbacher-disease; subventricular zone; central myelination; shaking pups; spinal-cord; spaniel dog; heat-shock; ng2 cells; proteins
Tags
IRIS
•
Formaldehyde
Nervous system effects
Found
Database search results
Web of Science
Screened
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Related to use in methodology
Reproductive and Developmental Effects
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Irrelevant species
Retroactive RIS import
Pre2013
Merged Litsearch Results 100912
Merged LitSearch Results ToxNet 101012
Merged LitSearch Additions 86 Reviews SCREEN
Web of Science Search 100412
2013
HCHON tox Ref Identification 022713
2015
FA DevRepro 072115
Irrelevant Species-Population Criteria
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