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1536426 
Journal Article 
Wound dressings loaded with an anti-inflammatory juca (Libidibia ferrea) extract using supercritical carbon dioxide technology 
Dias, AMA; Rey-Rico, A; Oliveira, RA; Marceneiro, S; Alvarez-Lorenzo, C; Concheiro, A; Junior, RNC; Braga, MEM; de Sousa, HC 
2013 
Yes 
Journal of Supercritical Fluids
ISSN: 0896-8446 
74 
34-45 
WOS:000315542300004 
N-Carboxybutyl chitosan (CBC), collagen/cellulose
(Promogran (R)) and hyaluronic acid-based (Hyalofill (R)) polymeric matrices/dressings were
loaded with an extract obtained from juca (Libidibia ferrea) and in order to develop wound
dressings endowed with anti-inflammatory activity. juca fruits were subjected to supercritical
fluid extraction (SFE) using CO2 at 25 MPa and 50 degrees C and the resulting extract was later
incorporated into the above referred wound dressings by a supercritical fluid
impregnation/deposition method (SSI). GC analysis revealed that the obtained SFE extract is
particularly rich in unsaturated (52%) and saturated (26%) fatty acids as well as in terpenoids
(13%) such as lupenone and gamma-sitosterol. Extract loading yields depended on the affinity of
the hydrophobic extract for the specifically employed wound dressing material and was almost 2-
fold greater for CBC than for the other two commercial wound dressings. The prepared extract-
loaded dressings were cytocompatible with RAW 264.7 macrophages (viability >85% at 24 h) and
down-regulated the expression of TNF-alpha. and IL-1 alpha pro-inflammatory cytokines as well as
the production of nitric oxide, which confirms the anti-inflammatory capacity of the employed
juca extract. Nevertheless, such effect was somehow counteracted by a pro-inflammatory activity
that was exhibited by CBC. Prepared dressings presented a wide range of water vapor ((2.9-14.7) x
10(14) kg/(s m Pa)) and oxygen permeability (150 up to 830 barrer) which make them potentially
suitable for the management of various wound types at different healing stages. (C) 2012 Elsevier
B.V. All rights reserved. 
Wound dressings; Libidibia ferrea extract (juca); Supercritical fluid extraction; Supercritical solvent; impregnation/deposition; Anti-inflammatory activity