Health & Environmental Research Online (HERO)


Print Feedback Export to File
1542060 
Journal Article 
Evaluation of antiulcer activity of chromanone fraction from Calophyllum brasiliesnse Camb 
Lemos, LM; Martins, TB; Tanajura, GH; Gazoni, VF; Bonaldo, J; Strada, CL; Silva, MG; Dall'oglio, EL; de Sousa Júnior, PT; Martins, DT 
2012 
Yes 
Journal of Ethnopharmacology
ISSN: 0378-8741 
ELSEVIER IRELAND LTD 
CLARE 
141 
1 
432-439 
English 
22425905 
WOS:000304571100054 
ETHNOPHARMACOLOGICAL RELEVANCE: Calophyllum brasiliense Camb. (Clusiaceae), popularly known as 'guanandi', is found in the tropical areas and swampy lands. The latex exuding from its bark is used in the treatment of gastric ulcer in folk medicine. Several active compounds have been isolated from its stem bark among them, are the chromanone acids. Therefore, this study aimed to evaluate antiulcer activity and probable mechanism(s) of action of a fraction containing a mixture of chromanone acids (BI), derived by column chromatography fractionation of the hexane extract of the stem bark of Calophyllum brasiliense (HECb), using experimental in vitro and in vivo models.

MATERIALS AND METHODS: Ulcer was induced by oral administration of ethanol (75%, v/v) and indomethacin (50mg/kg). Malondialdehyde, reduced glutathione and catalase activity was measured in stomach tissue after ethanol induced ulcer. In order to evaluate the effect of BI on nitric oxide, ulcer was induced by ethanol in l-NAME pretreated animals. Anti-Helicobacter pylori activity was verified in disk diffusion and broth microdilution in vitro assays, using cagA+ and vacA+ Helicobacter pylori strains.

RESULTS: BI prevented the gastric ulceration caused by ethanol and indomethacin treatments. Its gastroprotective mechanism in ethanol-induced ulcer was partly due to reduction of MDA and CAT levels in the gastric tissue. BI did not affect the GSH levels and its gastroprotective effect was not reversed by pretreatment with l-NAME. BI showed anti-Helicobacter pylori in the both assays.

CONCLUSION: The results indicate that BI is partly responsible for the HECb antiulcer and anti-Helicobacter pylori effects. 
Calophyllum brasiliense; Clusiaceae; Chromanone acids; Gastric ulcer; Anti-Helicobacter pylori activity