US EPA

1553542 

Journal Article 

Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants 

Lee, KH; Padzil, AM; Syahida, A; Abdullah, N; Zuhainis, SW; Maziah, M; Sulaiman, MR; Israf, DA; Shaari, K; Lajis, NH 

2011 

Yes 

Journal of Medicinal Plant Research
ISSN: 1996-0875 

5 

23 

5555-5563 

WOS:000297460400016 

The anti-inflammatory, antioxidant and anti-nociceptive
properties of six Malaysian medicinal plants, including Carica papaya, Musa acuminata, Oenanthe
javanica, Piper sarmentosum, Psophocarpus tetragonolobus and Sauropus androgynus were
investigated. The anti-inflammatory effects of the methanolic plant extracts were evaluated by
using Griess assay on nitric oxide inhibitory activity upon IFN-gamma/LPS stimulated RAW 264.7
cells. The antioxidant property and total phenolic content of the plant extracts were determined
by using DPPH radical scavenging assay and Folin-Ciocalteu's assay, respectively. Anti-
nociceptive activity of the plant extracts were evaluated by measuring the number of writhing
response of mice upon acetic acid induction. All plant species showed significant nitric oxide
(NO) inhibitory activity (IC(50) < 61 mu g/ml) without causing cytotoxicity to RAW 264.7 cells.
Besides, all six plants exhibited different degree of antioxidant activities (IC(50) value, 86.74
+/- 2.92 to 192.92 +/- 2.60 mu g/ml). The antioxidant activity might be due to the present of
phenolic compounds (34.20 to 50.01 mg GA/g DW samples). Moreover, all plant species suppress the
writhing response of mice at different degree of inhibition (10.65 to 43.12% inhibition) at
concentration tested of 200 mg/kg. Thus, this study validates the traditional medicinal uses of
the evaluated plant species in management of inflammation and free radical related disorders. 

Malaysian medicinal plants; anti-inflammatory; antioxidant; anti-nociceptive