US EPA

1554623 

Journal Article 

NMR-based Metabonomic Studies on Urine and Serum from Pr(NO3)(3)-treated Rats 

Liao Pei-Qiu; Xue Rong; Wu Yi-Jie; Pei Feng-Kui; Li Xiao-Jing 

2012 

Yes 

Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue
ISSN: 0253-3820 

40 

9 

1421-1428 

10.3724/SP.J.1096.2012.11185 

WOS:000313824600016 

The acute biochemical effects of Pr(NO3)(3) were studied by
NMR-based metabonomic approach on rats urine and serum. Male Wistar rats were administrated
intraperitoneally with Pr(NO3)(3) at doses of 2, 10 and 50 mg/kg body weight. Urine was collected
over a 168 h period after dosing and serum was collected at 48 h, 96 h and 168 h period. H-1 NMR
spectra of biofluids (urine and serum) were analyzed by principal components analysis. The
results revealed that Pr(NO3)(3) caused acute damages on rats liver and kidney, mainly in the
liver, and showed a significant dose-dependent relationship. The liver and kidney injury induced
by Pr(NO3)(3) was characterized by the variations of many endogenous metabolites, such as
citrate, succinate, a-ketoglutarate, creatinine, N-trimethyl dioxide, amino acids, lactate,
taurine and glucose. 

Praseodymium nitrate; Biofluids; Nuclear magnetic resonance; Biochemical effects; Pattern recognition; Metabonomics