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1561061 
Journal Article 
Effects of coenzyme Q10 and alpha-lipoic acid supplementation in fructose fed rats 
Ozdogan, S; Kaman, D; Simsek, BC 
2012 
Yes 
Journal of Clinical Biochemistry and Nutrition
ISSN: 0912-0009
EISSN: 1880-5086 
50 
2 
145-151 
22448096 
WOS:000302525900008 
has retraction 4800785 Editorial letter
This study was conducted to investigate the effects of
alpha-lipoic acid and coenzyme Q10 on plasma levels of lipids, asymmetric dimethylarginine,
oxidative stress in fructose fed rats which provide a model of dietary-induced insulin resistance
and to evaluate vascular changes developing in these rats by histologically. Male Sprague Dawley
rats were used in this study. The animals were divided into 4 groups. Group 1 did not receive any
medication and served as a control. Group 2 received a regular diet and water ad libitum and
fructose was administered as % 10 solution in drinking water. Group 3 received alpha-lipoic acid
(100 mg/kg/day) i.p. for 5 weeks and Group 4 received coenzyme Q10 (10 mg/kg/day) i.p. for 5
weeks. For determination of plasma asymmetric dimethylarginine, glutathione and malondialdehyde
levels, high-performance liquid chromatography system was used. Homeostatic model assessment as a
measure of insulin resistance was calculated. Lipid profile measurements were determined using
enzymatic assay on an Auto analyzer. The high fructose diet was significantly associated with an
increase in levels of plasma LDL, VLDL and total cholesterol and decrease in level of HDL
cholesterol. Plasma asymmetric dimethylarginine, malondialdehyde and glutathione levels were also
increase in these rats. alpha-lipoic acid or coenzyme Q10 supplementation was found to have some
positive effect on these parameters. These findings were also demonstrated by morphological
observation of the aorta. We demonstrated that administration of alpha-lipoic acid and coenzyme
Q10 notably suppresses oxidative and nitrative stress, hyperinsulinemia, insulin resistance
developing in fructose fed rats, a model of metabolic syndrome (MS). These positive effects of
alpha-lipoic acid or coenzyme Q10 can be attributed to its antioxidant activity. 
metabolic syndrome; coenzyme Q10; alpha-lipoic acid; ADMA; oxidative stress