US EPA

1568331 

Journal Article 

Isolation and characterization of an aerobic denitrifying Bacillus sp YX-6 from shrimp culture ponds 

Song, ZF; An, J; Fu, GH; Yang, XL 

2011 

1 

Aquaculture
ISSN: 0044-8486
EISSN: 1873-5622 

319 

1-2 

188-193 

10.1016/j.aquaculture.2011.06.018 

WOS:000294751500027 

In the present experiment, eighteen aerobic denitrifying
Bacillus strains were isolated by BTB plate technique. The Bacillus sp. strain YX-6 was selected
for the aerobic denitrification studies because it is highly effective in removing nitrite
compared with other strains of Bacillus sp. The results indicated that the strain YX-6 could
degrade the nitrite nitrogen (nitrite-N) from 10 mg/L to zero in 14 h and the nitrite-N
degradation rate was approximately to 100% at the DO concentration of 5.2-5.8 mg/L Furthermore,
the aerobic denitrification of the strain YX-6 was significantly higher than those of the
positive controls under different pH, temperature, nitrite and salinity concentrations (p <
0.05). The amplification of nitrite reductase confirmed that the strain YX-6 contained the nirS
gene. According to the morphological observation and 16SrRNA gene analysis, the strain YX-6 was
identified as Bacillus coagulans. (C) 2011 Published by Elsevier B.V. 

Aerobic denitrification; Bacillus coagulans; Nitrite; Nitrite nitrogen degradation rate; Aquaculture