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1572279 
Journal Article 
A superoxide anion biosensor based on direct electron transfer of superoxide dismutase on sodium alginate sol-gel film and its application to monitoring of living cells 
Wang, X; Han, Min; Bao, J; Tu, W; Dai, Z 
2012 
Yes 
Analytica Chimica Acta
ISSN: 0003-2670
EISSN: 1873-4324 
717 
61-66 
22304816 
WOS:000301566200007 
The direct electron transfer of superoxide dismutase (SOD)
was greatly facilitated by sodium alginate (SA) sol-gel film with the formal potential of 0.14 V,
which was just located between O-2(center dot-)/O-2 and O-2(center dot-)/H2O2. The preparation of
the SOD/SA modified electrode was simple without any mediators or promoters. Based on bimolecular
recognition for specific reactivity of SOD/SA toward O-2(center dot-) the SOD modified electrode
was utilized to measure O-2(center dot-) with good analytical performance, such as low applied
potential (0 V), high selectivity (no obvious interference), wide linear range (0.44-229.88 mu M)
and low detection limit (0.23 mu M) in pH 7.0 phosphate buffer solution. Furthermore, it could be
successfully exploited for the determination of O-2(center dot-) released from living cells
directly adhered on the modified electrode surface. Thus, the proposed O-2(center dot-)
biosensor, combining with the properties of SA sol-gel film, provided a novel approach for
protein immobilization, direct electron transfer study of the immobilized protein and real-time
determination of O-2(center dot-) released from living cells. (C) 2012 Elsevier B.V. All rights
reserved. 
Superoxide dismutase; Sodium alginate sol-gel film; Living cells; Superoxide sensor; Direct electron transfer 
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