Health & Environmental Research Online (HERO)


Print Feedback Export to File
1573647 
Journal Article 
Preparation and biological activity of saponin from Ophiopogon japonicus 
Xiong, SLi; Hou, D; Huang, Ni; Li, A 
2012 
6 
26 
1964-1970 
WOS:000307968900012 
Saponin from Ophiopogon japonicus was isolated and
preliminary identified by physical and chemical methods. The antioxidant and macrophages-
modulating activities of the saponin were also investigated employing various established systems
in vitro. The results of physical-chemical identification indicated that saponin was steroidal
saponin. It was observed that the scavenging activity of saponin on 2, 2-diphenyl-1-picry-
hydrazyl (DPPH) significantly increased in a concentration-dependent manner. The scavenging rate
of it on DPPH radical was 99.64% when the concentration of the saponin was up to 5 mg/ml. For
scavenging hydroxyl radical, it first increased and then decreased gradually with the increase of
concentration, and reached the strongest scavenging rate at the concentration of 2 mg/ml. Saponin
also exhibited prominent macrophages-modulating activity by the promotion of phagocytic capacity,
macrophage viability, nitric oxide (NO) production and interleukin-1 release. It follows that
saponin from O. japonicus can be explored as a novel and potential natural antioxidant and
immunostimulants. 
Ophiopogon japonicas; saponin; antioxidant activity; macrophages