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1574783 
Journal Article 
Effect of Atorvastatin on Fine Particle-induced Inflammation, Oxidative Stress and Endothelial Function in Human Umbilical Vein Endothelial Cells 
Zhao Jin-zhuo; Xie Yu-qua; Gao Zhi-yi; Jiang Rong-fang; Song Wei-min 
2010 
197-200 
WOS:000291569100133 
[Objective] Ambient fine particles contain a large number of constituents which can produce adverse effect on human health. The study was performed to explore the toxicity of organic extracts and water-soluble compositions of fine particles on human umbilical vein endothelial cells (HUVECs). [Methods] The exposure doses of organic extracts and water-soluble compositions were 100, 200 and 400)mu g/mL, respectively. Moreover, in this study, atorvastatin was used for intervention study on the possible toxicological mechanisms of fine particle constituents on HUVECs. HUVECs were stimulated by the 400 mu g/mL organic and soluble extracts of fine particles, respectively, followed by an intervention of astorvastatin in concentrations of 0.1 mu mol/L and 10 mu mol/L, respectively. Malondialdehyde (MDA), nitrous oxide (NO) and superoxide dismutase (SOD) were determined in cells. Meanwhile, the expression of interleukin-6 beta (IL-6) in HUVECs was assessed by reverse transcriptase polymerase chain reaction (RT-PCR) assay. [Results] The results showed that organic extracts and water-soluble compositions induced the increase of MDA level and the decrease of NO and SOD level, and enhanced the mRNA expression of IL-6 in HUVECs. Meanwhile, at the same exposure dose, water-soluble extracts induced more MDA and IL-6 in HUVECs compared with organic extracts. After adding the astorvastatin, the levels of MDA and the expression of IL-6 decreased in HUVECs, while the levels of NO and SOD increased. [Conclusion] The results indicated that atorvastatin attenuated fine particle-induced inflammation, oxidative stress and endothelial damage. The results suggested organic extracts and water-soluble compositions of fine particle could induce oxidative stress, inflammatory response and endothelial dysfunction in HUVECs. Water-soluble composition was more likely to induce inflammation, endothelial dysfunction and oxidative stress in comparison to organic extracts. The results indicated that the inflammation, oxidative stress and endothelial dysfunction might be the mechanisms of vascular endothelial dysfunction which mediated cardiovascular injury induced by different composition of fine particles. 
fine particle; cardiovascular; endothelial function