Health & Environmental Research Online (HERO)


Print Feedback Export to File
1592722 
Journal Article 
Cyanines as new fluorescent probes for DNA detection and two-photon excited bioimaging 
Feng, XJ; Wu, PL; Bolze, F; Leung, HW; Li, KF; Mak, NK; Kwong, DW; Nicoud, JF; Cheah, KW; Wong, MS 
2010 
1 
Organic Letters
ISSN: 1523-7060
EISSN: 1523-7052 
12 
10 
2194-2197 
English 
20405873 
WOS:000277531000007 
A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.