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1595995 
Journal Article 
A simple method for hyaluronic acid quantification in culture broth 
Song, JM; Im, JH; Kang, JH; Kang, DJ 
2009 
Yes 
Carbohydrate Polymers
ISSN: 0144-8617
EISSN: 1879-1344 
78 
3 
633-634 
English 
WOS:000269991800040 
The carbazole assay has been used for determination of the percentage of hyaluronic acid in biological fluids. However, it is difficult to measure the concentration of hyaluronic acid in culture broth because glucose and polysaccharides remaining after cultures can react with sulfuric acid and carbazole. The glucose and polysaccharide remnants must be completely removed in order to get the correct value for hyaluronic acid. The turbidity assay, another method for estimating the concentration of hyaluronic acid, is based on the formation of insoluble complexes between hyaluronic acid and cetyltrimethylammonium bromide. This method is very easy and fast compared with the carbazole assay. Because concentrations of hyaluronic acid measured by the turbidity assay were ranged around 100% of those measured by the carbazole assay, the content of hyaluronic acid in culture broth can be determined by the turbidity assay. The turbidity method also has the advantage of being safer than the carbazole assay. 
Hyaluronic acid; Culture broth; Carbazole; Turbidity assay; Cetyltrimethylammonium bromide