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1596329 
Journal Article 
Preparation of Cross-linked sodium hyaluronate gels with different molecular weights of PEG and research for its viscoelasticity and Enzyme-resistant properties in vitro 
Wang, WeiGuo; Cui, YuJia; Li, P; Zhao, YL; Gu, Ya'Nan; Wan, JiaLi 
2012 
Yes 
Advanced Materials Research
ISSN: 1022-6680 
Advanced Materials Research 
399-401 
1552-1558 
WOS:000310249301005 
Purpose To screen out the optimal conditions of cross linking reaction of preparing for an injectable cross-linked sodium hyaluronate gel(CHA-gel) with
higher resistance to hyaluronidase and research for its viscoelasticity and anti-enzyme
degradation properties. Methods The CHA hydrogels were prepared with different molecular weights
of PEG as cross-linking agent, such as PEG400, PEG1000, PEG6000, PEG10000, PEG20000. The optimal
preparing conditions were determined by single factor test and orthogonal experiment. The
Enzyme-resistant degradation properties in vitro of CHA-gels were analysed by carbazole and
spectrophotometry. Its viscoelasticity was also compared with natural HA-gel by Stabinger method.
Results the results of range analysis and variance analysis show that the pH of CHA solution and
the ratio of cross-linking agent to HA were significant factors. The optimal preparing conditions
of the parameters are 1.5% of HA, 0.001 mol/L NaOH, at 37 degrees C, reacting 4hr and 1:15
PEG20000/HA (g/g). Under these conditions, the CHA-gel has excellent Enzyme-resistant properties,
R=85.1%, an high percentage of enzyme-resistant property. Its viscoelasticity can reach 61.3x 10
(4)mPa.s, three times as much as natural HA-gel. Conclusion The CHA-gel with excellent
physicochemical properties can be prepared under the optimal conditions, which can set foundation
for developing better mechanical and Enzyme-resistant properties products of CHA-gel. 
cross-linked hyaluronate gels; different molecular weights of PEG; orthogonal experiment; enzyme-resistant degradation properties in vitro