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1600022 
Journal Article 
Development of highly specific fluorescence immunoassay and enzyme-linked immunosorbent assay for detection of dimethyl phthalate in water samples 
Zhang, M; Liu, B; Cong, Y; Liu, S; Hu, Y 
2011 
Yes 
Food and Agricultural Immunology
ISSN: 0954-0105
EISSN: 1465-3443 
22 
4 
297-309 
WOS:000299640800001 
A direct competitive fluorescence immunoassay (dc-FIA) and a direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the screening of dimethyl phthalate (DMP) in water samples were developed. The immunoassays utilise polyclonal antibodies against DMP raised in rabbits. The anti-DMP antibodies were linked to horseradish peroxidase (HRP) and fluorescein isothiocyanate (FITC). Under the optimal experimental conditions, the dc-ELISA has a linear working range of 0.1 2000 ng/ml (R(2) = 0.993) with a limit of detection of 0.09 ng/ml. In the dc-FIA, the linear working range was 0.05-30 ng/ml (R(2) = 0.996), and the limit of detection was 0.02 ng/ml, which is approximately four-fold more sensitive than the dc-ELISA using the same antibody and coating antigen. The results show low cross-reactivity with other structurally related compounds. The proposed methods are successfully applied to determine the DMP contaminants with a simple extraction procedure, and good recoveries were obtained. 
dimethyl phthalate; immunoassay; polyclonal antibody; water samples; fluoroimmunoassay; ELISA; foods; contamination