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HERO ID
1612972
Reference Type
Journal Article
Title
Chiral separation of two pairs of enantiomers of tadalafil by high-performance liquid chromatography
Author(s)
Gao, W; Zhang, Z; Li, Z; Liang, G
Year
2007
Is Peer Reviewed?
Yes
Journal
Journal of Chromatographic Science
ISSN:
0021-9665
Publisher
PRESTON PUBL INC
Location
NILES
Volume
45
Issue
8
Page Numbers
540-543
Language
English
PMID
18019566
DOI
10.1093/chromsci/45.8.540
Web of Science Id
WOS:000249313700015
Abstract
A high-performance liquid chromatographic method was developed for the chiral separation of a new selective phosphodiesterase 5 inhibitors, tadalafil and its three isomers. The chiral separation was performed on a Chiralpak AD column. The mobile phase was hexane-isopropyl alcohol (1:1, v/v). UV detection was at 220 nm. Baseline chiral separation for the four isomers was obtained within 30 min. Each of the resolutions of the two pairs enantiomers were more than 2.0. The limits of quantitation were 0.60, 0.90, 1.20, and 1.80 ng for (6R, 12aS), (6R, 12aR), (6S, 12aS), and (6S, 12aR) isomers, respectively. Relative standard deviation of the method was below 2% (n=5). The method is suitable in quality control.
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