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HERO ID
1613425
Reference Type
Journal Article
Title
Determination of quinolizidine alkaloids in Sophora tonkinensis by HPCE
Author(s)
Ding, PL; Yu, YQ; Chen, DF
Year
2005
Is Peer Reviewed?
1
Journal
Phytochemical Analysis
ISSN:
0958-0344
Volume
16
Issue
4
Page Numbers
257-263
Language
English
PMID
16042151
DOI
10.1002/pca.829
Web of Science Id
WOS:000230587800004
Abstract
A simple, rapid and reliable high-performance capillary electrophoresis method has been developed to determine quantitatively the alkaloid content of Sophora tonkinensis, a Chinese herb commonly known as shan-dou-gen. A total of seven quinolizidine alkaloids (cytisine, sophocarpine, matrine, lehmannine, sophoranol, oxymatrine and oxysophocarpine) could be readily separated within 15 min. The running buffer was 50 mM phosphate buffer (pH 2.5) containing 1% hydroxypropyl-beta-cyclodextrin and 3.3% isopropanol in water. The applied voltage was 25 kV, the capillary temperature was 25 degrees C, the detection wavelength was 200 nm and scopolamine butylbromide was used as internal standard. The method was used to analyse the chemical constituents of two commercial alternatives to shan-dou-gen. The alkaloid constituents of authentic shan-dou-gen gave a specific HPCE electropherogram that could be used to distinguish the drug from potential substitutes. Furthermore, the content of oxymatrine and the total content of the seven quinolizidine alkaloids could be used as quantitative markers in order to assess the quality of S. tonkinensis.
Keywords
high-performance capillary electrophoresis (HPCE); quinolizidine alkaloids; matrine; oxymatrine; quality assessment; Sophora tonkinensis; Leguminosae
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