US EPA

1616855 

Journal Article 

Determination of soybean phytosterols by reversed phase chromatography and gas chromatography-mass spectrometry 

Dai, J; Chen, SW; Fang, T; Wang, LP; Gu, XH; Tang, J 

2005 

Yes 

Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue
ISSN: 0253-3820 

33 

12 

1725-1729 

WOS:000234382300014 

The reversed phase chromatographic column Waters symmetry
C-18 (4.6 mm X 250 mm) and mobile phase consist of acetonitrile-isopropanol (95:5, V/V), suitable
to separate soybean phytosterols, was screened out. Effects of solvent ratio of mobile phase and
column temperature on the retention behaviors and resolutions of soybean phytosterols were
investigated. Four predominant phytosterols in soyasterol products extracted from soybean
deodorizer distillate including sitosterol and stigmasterol, campesterol and brassicasterol were
baseline resolved, and other unknown components were purified by high performance liquid
chromatography and then initially identified by gas chromatography-mass spectrometry as gamma-
sitosterol, epibrassicasterol, dihydrobrassicasterol. (24 beta-epimer of campesterol) and isomer
of stigmasterol. The sterol contents of soybean oil, deodorizer distillate and extracted
soyasterol products were determined under optimum chromatographic condition. The detection limits
for stigmasterol and beta-sitosterol were 14.4 ng and 10.6 ng, and the average quantitative
recoveries of the two phytosterols for soybean oil sample were 97.3% and 96.9% respectively. 

soyasterol; epimer; high performance liquid chromatography; gas chromatography-mass spectrometry